Common Name :
Silver Stain
Product Overview :
Silver Stain Kit is an exceptionally sensitive protein detection silver stain kit. It is suitable for staining both single dimension SDS-PAGE gels and two-dimensional (2D) gels of complex protein solutions, a common characterization technique used in proteomics.
Description :
Silver Stain Kit utilizes silver nitrate, which binds to selective amino acids on the proteins under weakly acidic or neutral pH conditions. The protein bound silver ions are reduced by formaldehyde at alkaline pH to form metallic silver in the gel.
Notes :
· All steps should be carried out at room temperature on an orbital shaker at 60 to 70 rpm.
· The gel should be stained in a glass or plastic tray, which has been cleaned with detergent and rinsed thoroughly.
· Clean, disposable gloves should be worn and changed before each step to prevent fingerprints on the gel.
· The volumes indicated in this procedure are for mini gels. The volumes should be tripled for large format (13 x 16 cm) gels.
· The staining process may be halted at the Fixing step by leaving the gel in the Fixing solution overnight if there is not enough time to complete the staining protocol.
Storage :
All kit components are stable at room temperature for at least 1 year.
Synonyms :
Silver Stain Kit; Fast Silver Stain Kit
Kit Components :
Silver solution
Silver Stain Sensitizer
Silver Stain Developer 1
Silver Stain Developer 2
Stop Solution
Sensitivity :
0.3ng BSA protein
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