Product Overview :
The G-LISA Rho activation assays are ELISA based Rho activation assays with which you can measure Rho activity in cells in less than 3 h. BK124 is very sensitive and has excellent accuracy between duplicate samples. For a more detailed introduction on G-LISA assays and a listing of other available G-LISA kits, see our main G-LISA page. The BK124 Rho activation assay kit measures the level of GTP-loaded RhoA only in cells. The level of activation is measured with absorbance set at 490nm.
Kit Components :
Reagent-Quantity -Storage96 well Rho-GTP binding plate: 12 strips of 8 wells, Desiccated 4°C; Anti-RhoA antibody Part: 2 tubes, lyophilized, Desiccated 4°C; Secondary antibody-horseradish peroxidase conjugate (HRP): 2 tubes, lyophilized, Desiccated 4°C; Rho control protein (constitutively active RhoA): 12 tubes, lyophilized, Desiccated 4°C; Cell Lysis Buffer: 1 bottle, lyophilized, Desiccated 4°C; Binding Buffer: 1 bottle, lyophilized, Desiccated 4°C; Wash Buffer: 1 bottle, lyophilized, Desiccated 4°C; Antigen Presenting Buffer: 1 bottle, 30 ml, Room temperature; Antibody Dilution Buffer: 1 bottle, lyophilized, Desiccated 4°C; HRP Detection Reagent A: 1 tablet, silver pack, Desiccated 4°C; HRP Detection Reagent B: 1 tablet, gold pack, Desiccated 4°C; HRP Stop Solution: 1 bottle, 8 ml, 4°C; Precision Red Advanced Protein Assay Reagent: (available as 500 ml size), 1 bottle, 100 ml, Room temperature; Protease Inhibitor Cocktail: 1 tube, lyophilized, Desiccated 4°C
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