Rubisco-Bisphosphate Carboxylase/Oxygenase (Rubisco) Activity Assay Kit

Cat.No. :

Kit-3893

Product Overview :

Significance: Rubisco is a key enzyme in plant photosynthesis, controlling CO₂ fixation and regulating carbon flow through the Calvin cycle and photorespiration. Its activity directly influences photosynthetic rate.
Principle: (1) Rubisco catalyzes the reaction of one molecule of RuBP with CO₂ to generate two molecules of 3-phosphoglycerate (PGA). (2) PGA is converted to glyceraldehyde-3-phosphate via phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase, coupled with NADH oxidation to NAD⁺. (3) The decrease in absorbance at 340 nm due to NADH oxidation reflects Rubisco carboxylase activity.

Size :

50t/48s

Storage :

Store at -20 °C. Shelf life: 6 months.

Detection method :

Ultraviolet Spectrophotometry