Product Overview :
S-Glutathionylated Protein Detection Assay Kit provides a convenient method for the direct visualization of S-glutathionylated proteins in whole (permeabilized) cells by flow cytometry and microscopy as well as avidin overlay analysis. This cell-based assay starts with the modification of protein free-thiols groups followed by enzymatic cleavage of any protein-S-glutathione (PSSG) adducts present in the sample. Biotinylation of the newly-formed protein free-thiols provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Reagents are provided to test three sets of 10 samples (most convenient) or up to thirty samples total at once if desired.
Description :
Glutathione (GSH) is a tripeptide (γ-glutamylcysteinylglycine) widely distributed in both plants and animals. GSH is involved in maintenance of protein sulfhydryl reduction status. The concentration of GSH ranges from a few micromolar in plasma to several millimolar in tissues such as liver. Mixed protein glutathionyl disulfides are a post translational protein modification of growing interest. Protein-S-glutathionylation may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins. PSSG detection methods can employ GSH adduct antibodies, GSH derivatives, and differential labeling systems based on the 'Biotin-Switch' method.
Stability :
1 year from the QC date provided on the Certificate of Analysis, when stored properly
Storage :
This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box. Once the kit has been opened, store the vials of PSSG Assay Buffer (10X) and PSSG Lysis Buffer at room temperature. Store the remaining components at -20°C.
Synonyms :
PSSG; S-Glutathionylated Protein Detection Assay Kit; S-Glutathionylated Protein; protein-S-glutathione; PSSG Detection Kit
Kit Components :
PSSG Assay Buffer (10X): 1 vial; RT;
PSSG Blocking Reagent: 3 vials; -20°C;
PSSG Lysis Buffer: 1 vial; RT;
PSSG Reduction Reagent: 3 vials; -20°C;
PSSG Labeling Reagent: 3 vials; -20°C;
PSSG Detection Reagent I (HRP): 1 vial; -20°C;
PSSG Detection Reagent II (FITC): 1 vial; -20°C
PDF