SRE Reporter Kit (MAPK/ERK Signaling Pathway )

Cat.No. :

Kit-1869

Product Overview :

The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.

Size :

500 reactions

Description :

The SRE Reporter Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.

Applications :

Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.Screen for activators or inhibitors of the MAPK/ERK signaling pathway.Study effects of RNAi or gene overexpression on the activity of the MAPK/ERK pathway.

Storage :

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Kit Components :

Reporter (Component A): SRE luciferase reporter vector + constitutively expressing Renilla luciferase vector; 500 µl (55 ng DNA/ µl); -20°C Negative Control Reporter (Component B): Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector; 500 µl (55 ng DNA/ µl); -20°CNote: These vectors are designed for transient transfection. They are NOT SUITABLE for transformation and amplification in bacteria.