Product Overview :
Simple, direct and automation-ready procedures for measuring starch concentrations find wide applications in research and drug discovery. Our starch uses a single Working Reagent that combines the enzymatic break down of starch and the detection of glucose in one step. The color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the starch concentration in the sample. This simple convenient assay is carried out at room temperature and takes only 30 min.
Description :
STARCH, chemical formula (C6H10O5)n, is a polysaccharide carbohydrate consisting of a large number of glucose units joined together by glycosidic bonds. All plant seeds and tubers contain starch present in the form of amylose and amylopectin. Starch is the most consumed polysaccharide in the human diet. Some starches are digested very quickly, and cause a rapid and large rise in blood sugar. Others are digested more slowly, and some starch, called resistant starch, is not digested in the small intestine at all, and thus causes little or no blood sugar rise.
Applications :
For quantitative determination of starch and evaluation of drug effects on starch metabolism.
Notes :
1. This assay is based on a kinetic reaction, the use of a multi-channel pipettor for adding the working reagent is recommended.
2. Interference. Interference. SH-group containing reagents (e.g., DTT, β-mercaptoethanol) may interfere with this assay and should be avoided in sample preparation.
Storage :
The kit is shipped on ice. Store all components at -20°C upon receiving. Shelf life: 6 months after receipt.
Kit Components :
Assay Buffer: 12 mL
Dye Reagent: 120 µL
Enzyme A: Dried
Enzyme B: 120 µL
Standard: 50 µL 50 mg/mL
Detection method :
OD570nm, or FL530/585nm
Compatible Sample Types :
Biological, agriculture, food etc
Features & Benefits :
Use as little as 10 μL samples. Linear detection range: 2 to 200 μg/mL starch for colorimetric assays and 0.2 to 20 μg/mL for fluorimetric assays.
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