Succinate Dehydrogenase (SDH) Activity Assay Kit

Cat.No. :

Kit-3896

Product Overview :

Significance of Measurement: Succinate dehydrogenase (SDH, EC 1.3.5.1) is a mitochondrial marker enzyme located on the inner mitochondrial membrane. It serves as a critical link between the electron transport chain and oxidative phosphorylation and also provides electrons for respiratory chains in prokaryotes.
Principle of Measurement: SDH catalyzes the oxidation of succinate to fumarate. The released hydrogen is transferred via phenazine methosulfate (PMS) to reduce 2,6-dichlorophenolindophenol (DCPIP), which exhibits a characteristic absorption at 600 nm. SDH activity is determined by monitoring absorbance changes at 600 nm.

Size :

100t/96s

Storage :

Store at -20 °C. Shelf life: 6 months.

Synonyms :

SDH Kit

Detection method :

Spectrophotometer/Microplate Reader