Yeast Mitochondria Isolation Kit

Common Name :

Mitochondria

Cat.No. :

Kit-0556

Product Overview :

Yeast Mitochondria Isolation Kit is used for isolating yeast mitochondria from yeast cell cultures.

Description :

Mitochondria are the power house of the cells because they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles: an outer membrane and a folded inner membrane called cristae. Isolated mitochondria is a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling. Yeast Mitochondria Isolation kit will enable fast and easy purification of mitochondria from yeast cells, utilizing yeast cell wall lysis and homogenization.

Applications :

Isolation of highly pure mitochondria from yeast cells.
Mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and for protein profiling.
Western blot

Usage :

For research use only (RUO)

Storage :

Store kit at -20°C, protected from light. Warm Buffer A and B to room temperature before use.

Kit Components :

Buffer A 50 ml
Buffer B 50 ml
1 M DTT 1 ml
Homogenization Buffer 50 ml
Lysis Enzyme Mix 200 μl
Storage Buffer 10 ml
Protease Inhibitor Cocktail ( Lyophilized ) 1 vial

Target Species :

Yeast

Compatible Sample Types :

Cell culture