Product Overview :
This Colorimetric Hydrogen Peroxide Assay Kit uses our unique IR peroxidase substrate to quantify hydrogen peroxide in solutions and cell extracts. Upon hydrogen peroxide oxidation the colorless IR generates an intense blue color product that is pH-independent from pH 4 to 10. The existing colorimetric hydrogen peroxide assays (from other vendors) often have severe sample interferences caused by the inherent absorption of biological samples. The near infrared absorption of IR product minimizes the assay background that since the biological samples rarely absorb light beyond 600 nm. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized 'mix and read' assay that is compatible with HTS liquid handling instruments.
Description :
Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for numerous oxidative stress-related states. It is involved in a number of biological events that have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. Perhaps the most intriguing aspect of H2O2 biology is the recent report that antibodies have the capacity to convert molecular oxygen into H2O2 to contribute to the normal recognition and destruction processes of the immune system. Measurement of this reactive species will help to determine how oxidative stress modulates varied intracellular pathways.
Storage :
Keep in freezer and avoid exposure to light.
Kit Components :
Component A: IR Peroxidase Substrate 1 vial
Component B: H2O2 1 vial (3% stabilized solution, 200 μL)
Component C: Assay Buffer 1 bottle (100 mL)
Component D: Horseradish Peroxidase 1 vial (20 units)
Component E: DMSO 1 vial (0.5 mL)
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