Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > 6-Phosphogluconic Acid Colorimetric Assay Kit

6-Phosphogluconic Acid Colorimetric Assay Kit

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Cat.No.:
Kit-2122
Product Overview:
6-Phosphogluconate assay kit can be used with a variety of sample types. In this assay, 6- Phosphogluconate is converted to Ribulose-5-Phosphate by 6-Phosphogluconate Dehydrogenase in the presence of NAD, to form NADH, which reduces a probe and generates strong absorbance at 450 nm. This 6-Phosphogluconate Assay Kit is simple, sensitive & easy to use and can detect 6-Phosphogluconate levels lower than 20 µM.
Size:
100 assays
Description:
6-Phosphogluconate (6-PGA) is an intermediate of both Pentose Phosphate Pathway (PPP) and Entner-Doudoroff Pathway. It is produced by the hydrolysis of 6-Phosphogluconolactone, catalyzed by 6-Phosphogluconolactonase. In the Pentose Phosphate Pathway, 6-PGA is utilized by 6-Phosphogluconate Dehydrogenase to generate ribulose-5-Phosphate and NADPH. These products are important for nucleic acid synthesis and various anabolic processes. In Prokaryotes, 6-Phosphogluconate is the main metabolite of EntnerDoudoroff pathway, and is converted into Pyruvate using both 6-Phosphogluconate Dehydratase and 2-Keto-3-Deoxyphosphogluconate aldolase. Recent studies show that long-term exposure to glucose perturbs the Pentose Phosphate Pathway, causes significant accumulation of 6-Phosphogluconate and impairs beta cell function. Measurement of 6-Phosphogluconate levels therefore is important for evaluating Pentose Phosphate Pathway, developing therapeutic approaches for diabetes research, and analyzing the Entner-Doudoroff Pathway in bacteria.
Applications:
Measurement of 6-Phosphogluconic Acid in various tissues/cells. Analysis of Pentose Phosphate Pathway and Entner-Doudoroff Pathway.
Target Species:
Mammalian
Storage:
Store the kit at –20°C, protected from light. Warm all Buffers to room temperature before use. Briefly centrifuge all small vials prior to opening.
Kit Components:
6-PGA Assay Buffer: 25 ml6-PGA Enzyme: 1 vial6-PGA Substrate Mix: 1 vial6-PGA Standard: 1 vial
Detection method:
Absorbance (OD= 450 nm)
Compatible Sample Types:
• Tissues: e.g. Liver, Kidney, Heart• Adherent or Suspension Cells: e.g. HeLa, Jurkat cells
Features & Benefits:
• Simple, fast and convenient assay• Can measure 6-PGA levels as low as 20 µM


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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