Acid Sphingomyelinase Activity Colorimetric Assay Kit is used to detect ASMase activity.
Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision"s Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/mL in a variety of samples.
Measurement of ASMase activity in various tissues/cell extracts.Screening of ASMase inhibitors.
For research use only (RUO)
Store the kit at -20°C, protected from light.
ASMase Assay Buffer I 20 mLASMase Assay Buffer II 15 mLASMase Substrate (Lyophilized) 1 vialASMase Enzyme Mix I (Lyophilized) 1 vialASMase Enzyme Mix II (Lyophilized) 1 vialCholine Standard (Lyophilized) 1 vialASMase Positive Control 10 µLASMase Probe (DMSO) 0.2 ml
Compatible Sample Types:
Animal tissues: brain, heart, kidney,etc.Cell culture: adherent or suspension cellsSerum