Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Metabolism Kits > Acid Sphingomyelinase Activity Colorimetric Assay Kit

Acid Sphingomyelinase Activity Colorimetric Assay Kit

"SMase" Related Products.
Product Overview:
Acid Sphingomyelinase Activity Colorimetric Assay Kit is used to detect ASMase activity.
Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision"s Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/mL in a variety of samples.
Measurement of ASMase activity in various tissues/cell extracts.Screening of ASMase inhibitors.
Target Species:
For research use only (RUO)
Store the kit at -20°C, protected from light.
Kit Components:
ASMase Assay Buffer I 20 mLASMase Assay Buffer II 15 mLASMase Substrate (Lyophilized) 1 vialASMase Enzyme Mix I (Lyophilized) 1 vialASMase Enzyme Mix II (Lyophilized) 1 vialCholine Standard (Lyophilized) 1 vialASMase Positive Control 10 µLASMase Probe (DMSO) 0.2 ml
Detection method:
Compatible Sample Types:
Animal tissues: brain, heart, kidney,etc.Cell culture: adherent or suspension cellsSerum

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.