Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Cellular Kits > CAPN1 Inhibitor (Human) Screening Kit

CAPN1 Inhibitor (Human) Screening Kit

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Cat.No.:
Kit-0151
Product Overview:
CAPN1 Inhibitor (Human) Screening Kit is used for screening human CAPN1 inhibitor.
Description:
Calpain 1 is calcium-dependent, non-lysosomal cysteine protease with low calcium requirement. It is expressed ubiquitously and in the brain, mainly localized to the cell body and dendrites of neurons and to a lesser extent in axons and glial cells. Hyperactivation of calpains is implicated in a number of pathologies associated with altered calcium homeostasis such as Alzheimer"s disease as well as secondary degeneration resulting from acute cellular stress following cerebral (neuronal) ischemia, traumatic brain injury and spinal cord injury. CAPN1 Inhibitor (Human) Screening Kit is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC possess very weak fluorescence; upon cleavage of the substrate by calpain 1, free AFC can be quantified fluorometrically (Ex/Em = 400/505 nm). In the presence of potent Calpain 1 inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Calpain 1 inhibitors. Inhibitor control (Calpain inhibitor 1, ALLN) is included to compare the efficacy of test inhibitors.
Applications:
Screening human Calpain 1 inhibitors
Target Species:
Human
Usage:
For research use only (RUO)
Storage:
Store kit at -80°C, protected from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge all small vials before opening. Read the entire protocol before performing the assay.
Kit Components:
Assay Buffer. Cap code: WM. 25 mlSubstrate. Cap code: red. 0.2 mlCalpain 1 Enzyme. Cap code: green. 0.2 mlInhibitor Control (Calpain Inhibitor 1, ALLN) [1 µM]. Cap code: blue. 0.5 ml
Detection method:
Fluorometric


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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