Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Cellular Kits > Caspase-2 assay Kit for drug discovery

Caspase-2 assay Kit for drug discovery

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Cat.No.:
Kit-0159
Size:
96 wells
Description:
Depending on their tissue of origin, the cells of caspase-2-deficient mice can be either more or less resistant to apoptosis induction than their wild-type counterparts. This would seem to imply that caspase-2 plays more the role of an apoptosis regulator than initiator. This role may be played in part via expression of the splicing isoforms caspase-2L (proapoptotic) and caspase-2S (anti-apoptotic). A possible exception, although perhaps one that proves the rule, is Salmonella-induced macrophage apoptosis, where caspase-2 may be acting with caspase-1 as a co-initiator. Research indicates that caspase-2L promotes apoptosis by causing release of proapoptotic mitochondrial proteins such as cytochrome c and Smac. Possible mechanisms include cleavage of Bid, direct, Bid-independent, action on the mitochondria and release of an unidentified nuclear factor. The Caspase-2 Assay Kit for Drug Discovery is a complete assay system designed to measure protease activity of caspase-2. Caspase-2 is unique among the caspases in its strong preference for pentapeptides over tetrapeptides as artifical substrates. The kit contains both a colorimetric substrate (Ac-VDVAD-pNA; Km= 53 µM) and a fluorogenic substrate (Ac-VDVAD-AMC; Km= 80 µM) based on the pentapeptide recognition sequence VDVAD. Cleavage of the p-nitroaniline (pNA) from the colorimetric substrate increases absorbance at 405 nm. The fluorescent assay is based on the cleavage of 7-amino-4-methylcoumarin (AMC) dye from the C-terminus of the peptide substrate which increases its fluorescence intensity at 460 nm. The kit is useful to screen inhibitors of caspase-2, a potential therapeutic target. A potent inhibitor Ac-VDVAD-CHO (aldehyde; Ki= 3.5 nM) is included for use as a control.
Applications:
Colorimetric detection, Fluorescence microscopy, HTS
Storage:
-80°C
Kit Components:
Caspase-2 enzyme (human) (recombinant), Substrate (Ac-VDVAD-pNA), Calibration Standard, AMC Substrate (Ac-VDVAD-AMC), AMC Calibration Standard, Inhibitor (Ac-VDVAD-CHO), Assay Buffer, 1/2-volume Microplate.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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