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Home > >> Services > Cell-free in vitro Protein Expression

Cell-Free in Vitro Protein Expression

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As a leading supplier for reagents in the biotechnology field, we understand the importance of convenient and easy-to-use systems for high level expression and sample purification. Creative BioMart has developed cell-free expression systems to meet the increasing demands for protein synthesis.

In vitro expression systems use an efficient coupled transcription and translation reaction to produce high yields of full-length, functional protein in both Prokaryotic system and Eukaryotic system. The time-consuming steps of cell-based protein production have been eliminated; now, in only two hours, we can produce protein starting from plasmid or linear DNA.
Prokaryotic system: E.coli Lysates
Eukaryotic system: Rabbit Reticulocyte Lysates, Wheat Germ system.

Two basic components are needed to accomplish cell-free protein expression: (1) the genetic template (mRNA or DNA) encoding the target protein and (2) a reaction solution containing the necessary transcriptional and translational molecular machinery. Cell extracts supply all or most of the molecules of the reaction solution, including:

  • RNA polymerases for mRNA transcription.
  • Ribosomes for polypeptide translation.
  • tRNA and amino acids.
  • Enzymatic cofactors and an energy source.
  • Cellular components essential for proper pro tein folding.
 


Applications for which cell-free protein expression are ideal:

  • Experiments to characterize protein-protein interactions and protein-nucleic acid interactions
  • Rapid and high-throughput expression of mutant or truncated proteins for functional analysis
  • Expression of mammalian proteins with proper glycosylation and native post-translational modifications (PTM)
  • Labeling of proteins with stable isotopes for structural analysis
  • Production of functional virons or toxic polypeptides
  • Analysis of components required for protein folding, protein stability or protein degradation
 

Welcome to contact us or send an email to info@creative-biomart.com for project quotations and more detailed information.
45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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