D-2-Hydroxyglutarate Dehydrogenase Fluorometric Assay Kit
Cat.No. : | Kit-2108 |
Product Overview : | D-2-Hydroxyglutarate Dehydrogenase Assay kit provides a quick and easy method for monitoring D2HGDH activity in a wide variety of samples. In this assay, D2HGDH converts D-2-Hydroxyglutarate into 2-Oxoglutarate and NADH, which further reduces PicoProbe to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 4 µU/µl of D2HGDH activity. |
- Specification
- Gene Information
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Size : | 100 assays |
Applications : | Measurement of D2HGDH activity in purified enzyme preparations, tissues and cells |
Storage : | Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.D2HGDH Assay Buffer: Bring to room temperature before use. Store at 4°C or -20°C.PicoProbe: Before use, thaw at room temperature. Store at -20°C. Use within two months.D2HGDH Substrate: Store at -20°C, stable for 6 months.D2HGDH Developer: Reconstitute with 220 µl Assay Buffer. Pipette up and down to dissolve completely. Store at -20°C. Use within two months.D2HGDH Positive Control: Reconstitute with 100 µl Assay Buffer and mix thoroughly. Aliquot and store at -70°C. Avoid freeze/thaw. Use within two months. Keep on ice while in use.NADH Standard: Reconstitute with 200 μl dH2O to generate 1 mM (1 nmol/μl) NADH Standard solution. Aliquot and store at –20°C. Use within two months. Keep on ice while in use. |
Products Types
◆ Recombinant Protein | ||
D2HGDH-02H | Recombinant Active Human D2HGDH Protein, His-tagged, Animal Free | +Inquiry |
D2HGDH-2180M | Recombinant Mouse D2HGDH Protein, His (Fc)-Avi-tagged | +Inquiry |
D2hgdh-2438M | Recombinant Mouse D2hgdh Protein, Myc/DDK-tagged | +Inquiry |
D2HGDH-1628D | Recombinant Danio Rerio D2HGDH Protein (56-533 aa), His-tagged | +Inquiry |
D2HGDH-4339H | Recombinant Human D2HGDH Protein, GST-tagged | +Inquiry |
◆ Lysates | ||
D2HGDH-7088HCL | Recombinant Human D2HGDH 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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