DNA Methylation Kit

• Cat.No.: Kit-0299
• Product Overview: DNA Methylation Kit is designed to methylate DNA from various samples.

Specification

• Applications: The DNA Methylation Kit features simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this kit makes it possible to amplify bisulfite converted DNA from as few as 10 cells or 50 pg DNA. DNA denaturation and bisulfite conversion processes are combined into a single step. This methylation kit streamlines the three step process of bisulfite conversion of non-methylated cytosine in DNA into uracil. In addition the methylation kit use innovative in-column desulphonation technology that eliminates otherwise cumbersome DNA precipitation steps while ensuring researchers consistent results every time. The kit has been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
• Storage: Store components of the kit at room temperatures up to the expiration date.After reconstitution with Storage Buffer, store Proteinase K at -20°C.