In the past many years, Creative BioMart has developed and manufactured high quality and leading edge proteins for biomedical and immunology research. One of our specialties is the production of unique immunoglobulin based chimeric fusion proteins using advanced cellular and molecular biological techniques. These reagents are used successfully in basic and applied research. Because of the high performance characteristics and quality the fusion proteins are widely recognized reagents and are highlighted in many scientific publications.
Non-lytic Ig-based Fusion Cytokines with Long Circulating Half-life
The potential clinical application of cytokines to modulate immune responses is highly demanded. Unfortunately, most cytokines have short circulating half-lives. Therefore, to facilitate the study of cytokine effects in vivo, a variety of non-lytic immunoglobulin-based cytokine fusion proteins have been created, in which a cytokine sequence had been genetically fused to the hinge, CH2 and CH3 regions of an immunoglobulin.
These non-lytic fusion proteins possess both the biological functions of the cytokine moiety and a prolonged circulating half-life determined by the Fc domain. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). These fusion molecules also have the promise of being minimally to negligibly immunogenic since they are made entirely from elements derived from the species to be treated.
Fc fusion proteins can play a role in dimerization, in vivo half-life, receptor activation and affinity for the cognate ligand. They have an advantage over soluble receptors as many receptors are only functional in a dimeric form; the dimeric receptor Fc can then mimic the activated form and enhance its affinity for the cognate ligand.
Fc chimeric proteins contain human-specific glycosylation and are suited for studies involving human proteins and cells. They can be used to neutralize the bioactivity of their cognate ligands in vitro and in vivo. They can also be used in studies to quantify receptor-ligand affinity by surface plasmon resonance.
Creative BioMart provides custom Fc fusion protein production services. For details, please contact us.