Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Detection Kits > Fluoro Lactate Detection Kit

Fluoro Lactate Detection Kit

   Share
"Lactate" Related Products.
Cat.No.:
Kit-0497
Product Overview:
In the assay, lactate oxidase (LOX) catalyzes the oxidation of L-lactate to pyruvate, along with the concomitant reduction of hydrogen peroxide (H2O2). The detection utilizes a non-fluorescent detection reagent, which is oxidized in the presence of horse radish peroxidase (HRP) and LOX to produce its fluorescent analog. Fluoro Lactate assay provides a reliable, sensitive fluorimetric method for the quantification of lactate in biological samples such as serum, plasma, urine, and tissue extracts.
Description:
Lactate is an intermediate product of carbohydrate metabolism. Of the two forms of Lactate, D-and L-, the L-lactate is predominant isomer found in biological systems. L-lactate is formed during the anaerobic glycolysis by conversion of pyruvate to L-lactate by lactate dehydrogenase. Lactate level is an indicator for tissue oxygen demand and utilization. Abnormally high lactate levels are associated with diseases such as diabetes and lactate acidosis. Fluoro Lactate assay is a lactate oxidase-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and tissue extract.
Usage:
1. For research use only. Not for use in diagnostic procedures.2. Practice safe laboratory procedures by wearing protective clothing and eyewear.3. Glutathione (reduced form GSH) may interfere with the assay.See Technical note 3.
Storage:
1. Short term (several weeks): Kit: see individual components.
Kit Components:
Reagent-Storage Temperature1. Lactate Standard 4mM: 500µl 2-8°C;2. Horseradish Peroxidase: 18.9 Units 2-8°C;3. 5X Reaction Buffer: 25 mL 2-8°C;4. Detection Reagent: 1 Vial -20°C;5. Reaction Enzyme Mix: 1 Vial-1.1mL -20°C
Features & Benefits:
1. Highly effective and stable fluorescent assay for L-lactate.2. Simple and fast assay-add the reagent directly to your experimental samples. Plate can be incubated and read in 15-30 min.3. Works for serum, plasma and tissue extract.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.