Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > GCN5 Chemiluminescent Assay Kit

GCN5 Chemiluminescent Assay Kit

   Share
"GCN5" Related Products.
Cat.No.:
Kit-1736
Product Overview:
GCN5 (also known as KAT2A) is involved in various cellular events, and its dysfunction is linked to a number of human diseases including cancers and diabetes. GCN5 is a histone acetyltransferase that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B by promoting ubiquitination of the NF-kappaB subunit RelA in a HAT-independent manner.
Size:
96 reactions
Description:
The GCN5 Chemiluminescent Assay Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of GCN5. The GCN5 Chemiluminescent Assay Kit is based on the GCN5 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader.
Applications:
Great for studying enzyme activity and screening small molecular inhibitors for drug discovery and HTS applications.
Storage:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
GCN5 human recombinant enzyme: 10 µg; -80°C Acetyl CoA (1 mM): 500 µl; -20°C 10X HAT assay buffer: 10 ml; -20°C Primary antibody 21: 25 µl; -20°C Secondary antibody 2: 12 µl; -20°C HRP chemiluminescent substrate A (transparent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C Blocking buffer: 25 ml; +4°C White microplate (or strips) precoated with Neutravidin and Histone peptide: 1 plate; Room temp.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.