GCN5 (also known as KAT2A) is involved in various cellular events, and its dysfunction is linked to a number of human diseases including cancers and diabetes. GCN5 is a histone acetyltransferase that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B by promoting ubiquitination of the NF-kappaB subunit RelA in a HAT-independent manner.
The GCN5 Chemiluminescent Assay Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of GCN5. The GCN5 Chemiluminescent Assay Kit is based on the GCN5 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader.
Great for studying enzyme activity and screening small molecular inhibitors for drug discovery and HTS applications.
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
GCN5 human recombinant enzyme: 10 µg; -80°C Acetyl CoA (1 mM): 500 µl; -20°C 10X HAT assay buffer: 10 ml; -20°C Primary antibody 21: 25 µl; -20°C Secondary antibody 2: 12 µl; -20°C HRP chemiluminescent substrate A (transparent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C Blocking buffer: 25 ml; +4°C White microplate (or strips) precoated with Neutravidin and Histone peptide: 1 plate; Room temp.