Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > Assay Kits > Metabolism Kits > Glucose-1-Phosphate Colorimetric Assay Kit

Glucose-1-Phosphate Colorimetric Assay Kit

"G1P" Related Products.
Product Overview:
Glucose-1-Phosphate Assay Kit (Colorimetric) is a quantitative colormetric determination of glucose-1-phosphate at 450 nm.
Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P → G1P), UDPG-pyrophosphorylase (G1P → UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] → UDP + glycogen[n+1]). Glucose-1- phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties. In Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. Glucose-1-Phosphate Assay Kit (Colorimetric) is rapid, sensitive & easy to use & can detect 1µM to 10 mM G1P. This G1P assay kit can be used for a variety of sample types.
Measurement of Glucose-1-Phosphate in various tissues/cells.Analysis of glucose metabolism and cell signaling in various cells.Screening anti-diabetic drugs.
Target Species:
For research use only (RUO)
Store kit at -20°C, protected from light. Warm all Buffers to room temperature before use. Briefly centrifuge all small vials prior to opening.
Kit Components:
G1P Assay Buffer 27 mLG1P Enzyme Mix (Lyophilized) 1 vialG1P Developer (Lyophilized) 1 vialG1P Substrate Mix (Lyophilized) 1 vialG1P Standard (Lyophilized) 1 vial
Detection method:
Compatible Sample Types:
Animal tissues: Liver, muscle and heart etc.Cell culture: Adherent or suspension cells.

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.