Glucose Assay Kit is a quantitative colorimetric glucose determination at 630 nm.
Glucose (C6H12O6) is a ubiquitous fuel molecule in biology. It is oxidized through a series of enzyme-catalyzed reactions to form carbon dioxide and water, yielding the universal energy molecule ATP. Due to its importance in metabolism, glucose level is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism.
Direct Assays: glucose in biological samples (e.g. serum and plasma).Drug Discovery/Pharmacology: effects of drugs on glucose metabolism.Food and Beverages: glucose in food, beverages etc.
For research use only (RUO)
Store the reagent at room temperature and standard at -20°C, respectively. Shelf life: 12 months after receipt.
Reagent 50 mLStandard 1 mL 300 mg/dL
Compatible Sample Types:
Biological Sample, Plasma, Serum
Features & Benefits:
Sensitive and accurate: Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (39 µM) to 300 mg/dL (16.6 mM) glucose in 96-well plate.Simple and convenient: The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath.Improved reagent stability: The optimized formulation has greatly enhanced the reagent and signal stability.Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum and plasma samples.