Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Glucose Cellular Uptake Measurement Fluorometric Kit

Glucose Cellular Uptake Measurement Fluorometric Kit

   Share
"Glucose" Related Products.
Cat.No.:
Kit-0374
Product Overview:
A complete reagent set to measure 2DG in cells after cell lysis by sonication.
Description:
Insulin is a hormone that lowers the blood glucose level and is known to help glucose enter cells through an effect that enhances the activity of glucose transport, primarily in insulin-sensitive organs cells, such as adipocytes and skeletal muscles.The effect of insulin and other growth factors on cells is often assessed in the laboratory by measuring glucose uptake activity. Typically, glucose uptake is measured using 3-O-Methyl-D-Glucose or 2-deoxyglucose (2DG) labeled with radioactive 3H or other isotope. However, the use of radioactive isotopes is not available to all labs and is subject to many restrictions. This assay kit was developed to provide a simple, rapid and convenient means to measure cellular glucose uptake without the use of radioisotope.This assay utilizes the glucose analog 2-deoxyglucose in place of glucose. Like glucose, 2DG taken up by cells is rapidly phosphorylated by hexokinase to 2-deoxyglucose-6-phosphate (2DG6P). However, unlike glucose, 2DG6P is not further metabolized and accumulates in cells. Using the provided reagents, cell lysates are then assayed for 2DG6P levels in a coupled enzymatic re-dox reaction that produces a fluorescent signal whose intensity is proportional to the amount of accumulated 2DG6P. 2DG levels in cell lysate samples are thus calculated by comparing their fluorescence intensity to a standard curve produced with known amounts of 2DG6P.
Storage:
Store kit at -20°C until expiration date printed on the label, protected from light.
Kit Components:
1. 2DG6P Solution (1 mM): 500 μL 3 x 3 ml;2. Sample Diluent Buffer Concentrate (100x): 5 ml x 1 vial;3. Substrate Buffer: 9 mL x 1 vial;4. Fluorescent Substrate :120 μL x 1 vial;5. Enzyme Solution: 270 μL x 1 vial


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.