Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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L-Malic acid Colorimetric Assay Kit

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Cat.No.:
Kit-2065
Product Overview:
Enzymatic and rapid colorimetric test for the determination of L-malic acid. Based on the spectrophotometric measurement of INT-formazan formed through the combined action of L-malate dehydrogenase (L-LDH), aspartate aminotransferase (AST) and diaphorase.
Size:
5 x 10 tests
Description:
L-Malic acid is a relevant component of the citric acid cycle that is found in animals, plants and microorganisms. It is one of the most important fruit acids found in nature and it is the acid present in highest concentrations in wine. Microbial decomposition of L-malic acid leads to the formation of Llactate; this can be a desirable reaction in the wine industry, where the level of L-malic acid is monitored, along with Llactic acid, during malolactic fermentation.
Applications:
This rapid and simple colorimetric method is used for the determination of L-malic acid (L-malate) in a wide range of matrices. Although specially developed for quantification of L-malic acid in wine industry, this kit is also adequate to Lmalic acid measurement in other foodstuffs such as fruit juice, beer, bread, fruit and vegetable products, as well as in cosmetics, pharmaceuticals and biological samples. Simple, robust, and accurate, this assay can be performed using an inexpensive visible spectrophotometer.
Kit Components:
Solution 1. Glycylglycine buffer (60 mL, 0.25 M, pH 10.0) plus L-glutamate (0.25 M), Triton X100 (1.25% V/V) and sodium azide (0.02% w/v) as a preservative. Stable for 2 years at 4 °C. Mixture 2 (5 x). NAD+ (30 mg) plus INT (1.1 mg) and FAD (75 ug). Stable for 5 years at -20 °C. Dissolve content of 1 bottle in 12 mL of Solution 1. Stable for at least 48 h. Suspension 3. Aspartate aminotransferase (AST/GOT), LMalate dehydrogenase (LMDH) and Diaphorase in 3.2 M ammonium sulphate (2.2 mL). Stable for 2 years at 4 °C. Swirl bottle before use. Solution 4. L-Malic acid standard solution (5 mL, 0.4 mg/mL). Stable for >2 years at 4 °C. This standard solution can be used when there is some doubt about the method accuracy. Powder 5: PVPP (polyvinylpolypyrrolidone; 10 g)
Detection method:
Colorimetric method
Compatible Sample Types:
Wine, beer, fruit juices, milk, dietetic foods, bread, jam, honey, ice-creams, fruit and vegetables, pharmaceuticals, cosmetics and biological samples.
Features & Benefits:
Simple and robust formatUse of inexpensive visible spectrophotometerRapid reaction
Sensitivity:
Reaction volume: 3.04 mLRange: 8-800 mg/LDetection limit: 8 mg/L


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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