Lipase Activity Assay Kit (Colorimetric) is a product where lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by via monitoring a linked change in the OxiRed probe absorbance (OD=570nm).This assay is rapid, simple, sensitive, and reliable for activity screening of lipase. This kit detects lipase activity as low as 0.02mU per well. For HTP analysis, we recommend Lipase Detection Kit II (Colorimetric) or Lipase Detection Kit III (Fluorometric).
Store kit at -20ºC in the dark immediately upon receipt. Kit has a storage time of 1 year from receipt, providing components have not been reconstituted.Refer to list of materials supplied for storage conditions of individual components. Observe the storage conditions for individual prepared components in section 5.Aliquot components in working volumes before storing at the recommended temperature. Reconstituted components are Stable for 2 months.
Components Identifier 100 testsAssay Buffer WM 1 x 25mlEnzyme Mix (lyophilized) Green 1 vialGlycerol Standard Yellow 1 x 0.2mlLipase positive control Purple 1 vialLipase Substrate Blue 1 vialOxiRed Probe Red 1 x 0.2ml
Compatible Sample Types:
Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media