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You Position:  Home >> Services >> Membrane Protein Services >> Lipid-based Protein Immobilization

Lipid-based Protein Immobilization
The Lipid-based Protein immobilization technology (LiPi), developed in collaboration with pharmaceutical and academic groups, enables immobilization of native membrane proteins in the form of proteoliposomes, generated directly from cells or tissue.

The LiPi™ technology is based on the use of proprietary surfaces and sample preparation protocols designed to retain the natural environment around the membrane proteins. The membrane proteins can be subjected to a variety of protocols in a highly controlled manner, to provide effective and precise preparation of samples for downstream analysis.
LiPi™ FlowCell is the first product based on the LiPi technology and is used in applications such as functional studies, absolute quantification, epitope identification and sequencing studies amongst others. Additional products are in development, where other formats will complement LiPi™ FlowCell and allow straightforward expansion of experimental protocols and applications.

Applications:

  • • Ligand binding studies
  • • Antigen epitope identification
  • • Quantitative proteomics
  • • Protein sequencing with multi-step digestion
  • • Easy and efficient membrane protein profiling

Key Features:

  • • Developed protocols for membrane preparations
  • • Extensive knowledge in membrane proteomics
  • • Unique in-house developed LiPi™ technology for sample preparation prior MS analysis
  • • Optimized protocols for a wide variety of applications
  • • State-of-the-art MS facility
  • • Experience in analyzing complex membrane protein samples
  • • Highly skilled bioinformatics group specialized in interpreting complex membrane protein data
  • •In-house developed protein annotation database

 

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