The Fluoro NAD/NADH detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH.
The role of pyridine nucleotides (NAD/NADH) metabolism in health, is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation and are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling. This suggests that either the enzymes or NAD could be an important therapeutical target 1.
1. Detection of NAD/NADH activity in cells or tissue extracts.2. Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair. senescence, endocrine signaling and life span.4. NAD/NADH detection in Bacterial, fungal and plant cells.
1. For Research use only. Not for use in diagnostic procedures.2. Practice safe laboratory procedures by wearing protective clothing and eyewear.3. The fluorescent product of the detection reagent is not stable in the presence of thiols (DTT or 2-mercaptoethanol). Keep these reactants below 10mM. 4. Once the vial of Part 4018 (NADH Detection reagent) is opened, it is important that low lighting conditions be used while aliquoting as well as performing the experiment. Direct and prolonged light exposure may increase the background, resulting in compromised linearity.
1. Long Term Storage: Store contents as labeled;2. Upon Arrival: Various-Please see kit components above for specific storage conditions.3.: Enzyme Mix,: NADH Standard and: NADH Detection reagent. Upon arrival store below -20°C and away from light.4. The rest of the components should be stored at 2-8°C until first use.
Reagent-Storage Temperature1.Enzyme Mix, Storage: -20°C; 2.NADH Detection Reagent, Storage: -20°C; 3.NADH Standard, Storage: -20°C; 4.NAD/NADH Reaction Buffer, Storage: 2-8°C; 5.NAD/NADH Lysis Solution, Storage: 2-8°C; 6.NAD Extraction Buffer, Storage: 2-8°C; 7.NADH Extraction Buffer, Storage: 2-8°C; 8.NADH Standard Diluent, Storage: 2-8°C
Features & Benefits:
1. Detection of NAD/NADH content in cells or tissue extracts.2. Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair, senescence, endocrine signaling and life span.3. NAD/NADH detection in Bacterial, fungal and plant cells.4. Fluorescent 96 well Plate Reader Readout (excitation: 530-570nm and emission at 590-600nm).