"Phosphate" Related Products

Phosphate Fluorometric Assay Kit

Cat.No.: Kit-0687
Product Overview: Phosphate Assay Kit (Fluorometric) provides a sensitive, easy, quick means of assessing phosphate over a wide range of concentrations. In the assay, inorganic phosphate reacted with maltose to produce glucose in the present of a special enzyme. The glucose is specifically oxidized to generate a product that reacts with the OxiRed probe to generate fluorescence (Ex/Em=535/587nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples. Glucose background can be eliminated by doing a glucose control.
Size: 100 tests
Description: Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in variety samples or monitor Pi changes during kinase and phosphatase reactions.
Applications: Functional Studies more details
Storage: Store at -20°C.
Kit Components: Components: Identifier; Converter: Blue; Developer: Green; Phosphate Assay Buffer: WM; Phosphate Standard: Yellow; Phosphate Substrate: Purple; PicoProbe (in DMSO): Blue
Compatible Sample Types: Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
Assay time: 2h 00m
Sensitivity: >0.4 µM

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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