Creative BioMart has a high throughput protein microarray technique that enables measurements of protein expression levels in hundreds of biological samples. Proteins extracted from cell lysates, bio-fluids or fixed-tissues are immobilized as spots in rows on a solid support. Each micro-spot holds the entire proteome of a sample. Highly specific antibodies are used to simultaneously screen these spots for the level of distinct target proteins.
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Advantages:
- High throughput method using automation
- Microvolumes of sample (5-10 µl)
- Convenient cost
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Key advantages against Western Blot analyses:
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Creative BioMart Protein Expression Microarray |
Western blot |
| Speed and costs |
-until 500 sample per slide
-until 16 antibodies per slide
-Low amount of sample needed
(5ul to test hundreds of targets simultaneously) |
-until 10 sample per gel
-1 antibody per gel
-Significant amount of sample needed
(10ul for each target to test) |
| Data quality |
-Accuracy 5%
-High reproducibility (C.V. around 5%) |
-Accuracy 20%
-Medium reproducibility (C.V. around 25%) |
Price on request, please contact us for a quote or for further information.