Protein SUMOylation Assay

Protein SUMOylation Assay

Creative BioMart provides protein SUMOylation assay system for generating SUMOylated proteins in vitro. We provide carboxy-terminal SUMO-1, SUMO-2 or SUMO-3 peptide substrates which are linked covalently to specific lysine residues on the target protein via isopeptide bonds.

Western blot analysis of in vitro SUMOylation of p53 protein by wild-type and mutated isoforms of SUMO-1: The two Western blots were incubated with p53 antibody (1:5000 dilution) and SUMO-1 antibody (1:4000 dilution). The p53 protein is SUMOylated only by the wild-type SUMO-1 protein.

Applications

  • Investigation of SUMOylation effects on transcription factor activity
  • Understanding the role of SUMOylation in the regulation of cellular processes
  • Identification of novel proteins as targets for SUMO

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