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You Position:  Home >> Services >> Controlled Randomization Service

Controlled Randomization Service
The GenePowerTM Controlled Randomization technology allows you to accurately direct the effective frequency of random nucleotide substitutions. And unlike error-prone PCR, you decide exactly which part(s) of the gene are affected—randomize the entire open reading frame or confine the

Randomized constructs are rigorously controlled to help ensure that the unchanged portions of the sequence are unaffected and that randomized portions contain the distribution of nucleotides you require to encode the amino acid composition you're looking for.

Applications
• Diversify antigen-binding sites of antibodies
• Improve physicochemical protein properties such as solubility, heat stability, and activity in industrial environments
• Enhance function, such as promoter activity and enzymatic properties
• Optimize antibody affinity (in vitro affinity maturation)
• Humanize antibodies
• Prolong half-life of therapeutics for in vivo environments
• Improve enzymes for industrial use
• Modify enantioselectivity of enzymes
• Induce changes to alter proteins from patented sequences

Advantages
• Library diversity of up to 1011 variants
• Precise selection of the average mutation rate (number of changes per construct)
• Random mutagenesis throughout construct, or limited to defined positions
• Transition/transversion ratio of 1:2
• Fewer silent mutations
• Low ancillary mutation rate
• Fast production times

 


 

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