Ras Activation Kit
| Cat.No. : | Kit-0762 |
| Product Overview : | Ras Activation Kit provides a rapid, cost-effective and reliable tool for the detection and semi-quantitative analysis of the cellular activation state of the three prototypical Ras proteins K-Ras, N-Ras and H-Ras (collectively referred to as Ras). Active Ras, i. e. Ras complexed to GTP, is purified from cell extracts via a one-step batch affinity purification step employing GST-RBD, an affinity probe that selectively interacts with the active, GTP-bound Ras conformation in loss of the inactive Ras-GDP complex. The kit further includes recombinant H-Ras. K-Ras and N-Ras proteins serve dual roles: 1. They may be used as Western Blot controls for Ras isoform-specific immunodetection2. Upon loading with GDP or GTP-γ-S (also included in the kit), Ras-GDP or Ras-GTP-γ-S proteins can be used to "spike" cell extracts thus serving as internal assay controls of signal specificity. |
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| Size : | 1 kit |
| Applications : | Functional Studies more details |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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