Recombinant Ferret TNF Protein

Cat.No.: TNF-1022F
Product Overview: Recombinant mature form of Ferret (Mustela putorius furo) TNFα (A3FBF1) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
Source: E. coli
Species: Ferret
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is typically 50-200 pg/ml.
Molecular Mass: The recombinant Ferret TNFα consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. The apparent molecular mass of the recombinant protein is approximately 16 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: TNF tumor necrosis factor [ Mustela putorius furo ]
Official Symbol: TNF
Synonyms: DIF; TNFA; TNF-a; TNFSF2; TNFalpha; TNF alpha; tumor necrosis factor alpha
Gene ID: 101687148
mRNA Refseq: NM_001310183
Protein Refseq: NP_001297112
MIM:
UniProt ID:

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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