Recombinant Ferret TNF Protein
Recombinant mature form of Ferret (Mustela putorius furo) TNFα (A3FBF1) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is typically 50-200 pg/ml.
The recombinant Ferret TNFα consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. The apparent molecular mass of the recombinant protein is approximately 16 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.