Recombinant Human ATF3, fused with His-SUMO tag, was expressed in E. coli.
This protein binds the cAMP response element (CRE) (consensus: 5"-GTGACGT[AC][AG]-3"), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
20mM Tris.Cl, 50mM NaCl, 20% Glycerol, pH8.0.
38.2kDa(including fusion tag)
342aa(including fusion tag)
WB, ELISA, IP, antibody production, protein array
Store at -80°C and avoid repeated freeze-thaw cycles.