Active Recombinant Human MMP13 protein

• Cat.No.: MMP13-27H
• Product Overview: Recombinant Human MMP13 was expressed in E. coli.

Specification

• Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
• Source: E. coli
• Species: Human
• Form: The protein Solution (100 µg/ml) in 0.05M Tris-HCl buffer, pH 7.6, containing 0.2M NaCl, 5mM CaCl2, 20µM ZnSO4 and 0.1% BSA and NaN3.
• Bio-activity: The specific biological activity measured by the ability of fibrin lysis in agarose plate was found to be 80000U/mg.
• Molecular Mass: 27 kDa
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: MMP-13 although stable at 4 centigrade for 1 week, should be stored desiccated below -18 centigrade. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: MMP13 matrix metallopeptidase 13 (collagenase 3) [ Homo sapiens ]
• Official Symbol: MMP13
• Synonyms: MMP13; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); collagenase 3; CLG3; MMP-13; MANDP1
• Gene ID: 4322
• mRNA Refseq: NM_002427
• Protein Refseq: NP_002418
• MIM: 600108
• UniProt ID: P45452
• Chromosome Location: 11q22.3
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem
• Function: calcium ion binding; calcium-dependent protein binding; fibronectin binding; low-density lipoprotein particle receptor binding; metalloendopeptidase activity; peptidase activity; zinc ion binding