Recombinant Human CTSK was expressed in Insect cells.
Cathepsin K, a member of the papain family of cysteine proteases, acts upon proteins such as collagen, AL amyloid, kinin and elastin. It is a lysosomal protease expressed primarily in osteoclasts, but also in other cell types such as macrophages. It functions in bone remodeling and is implicated in disease states such as atherosclerosis, arthritis and pycnodysostosis.
Liquid. In 27.8µl 20mM sodium phosphate, pH 6.9, 500mM NaCl, 1 mM EDTA.
To activate proenzyme, add 32.5mM NaOAc, pH 3.5, to 20% of procathepsin K volume (e.g., 5µl procathepsin K + 1µl 32.5mM NaOAc, pH 3.5), and incubate at room temperature for 25-30 minutes, or at +4 centigrade for approximately seven hours.
Useful tool to study enzyme kinetics, cleave target substrates and screen for inhibitors.
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80 centigrade.
Use/Stability: -80 centigrade. After initial defrost, aliquot product into individual tubes and refreeze at -80 centigrade. Avoid repeated freeze/defrost cycles and extended periods unfrozen. This enzyme is stable when stored as received under the above conditions. Procedures such as dilution of the enzme followed by refreezing could lead to loss of activity.
Long Term Storage: -80 centigrade