Recombinant Mouse Muc1(Ser410~Ala525) fused with His/GST tag at the N-terminus was expressed in E. coli.
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted Molecular Mass: 45.0kDa
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- S PQLSVGVSFF FLSFYIQNHP FNSSLEDPSS NYYQELKRNI SGLFLQIFNG DFLGISSIKF RSGSVVVEST VVFREGTFSA SDVKSQLIQH KKEADDYNLT ISEVKVNEMQ FPPSA
<1.0EU per 1µg (determined by the LAL method)
SDS-PAGE; WB; ELISA; IP.
Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitute in sterile PBS, pH7.2-pH7.4.