Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Inquiry now !
* E-mail Address:
* Service & Products Interested:
Project Description:
* Verification code:
Please input "biomart" as verification code.
Submit

SERVICES
Your Position:  Home >> Services >> SUMO-tag Protein Production

SUMO-tag Protein Production

Share

Small Ubiquitin-like Modifier (or SUMO) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. As ubiquitin, SUMO is covalently linked to its substrates via amide (isopeptide) bonds formed between its C-terminal glycine residue and the ?-amino group of internal lysine residues. The enzymes involved in the reversible conjugation of SUMO are similar to those mediating the ubiquitin conjugation.

It has been found that SUMO as an N-terminal fusion partner enhances functional protein production in prokaryotic and eukaryotic expression systems, based upon significantly improved protein stability and solubility.In addition, the tertiary structure of the SUMO protein is specifically recognized and cleaved by a ubiquitin-like protein-processing enzyme, SUMO Protease resulting in the production of native protein.

SUMO-tag Protein Expression System developed by Creative BioMart utilizes SUMO to allow expression, purification, and generation of native proteins in both prokaryotic and eukaryotic expression systems. Following the expression and purification of the fusion protein, the SUMO-tag can be cleaved by specific (SUMO) proteases via their endopeptidase activity in vitro to generate the desired N-terminus of the released protein partner.

Advantages:

  • Tag design: SUMO tag
  • Purification: Immobilized metal ion affinity chromatography (IMAC)
  • Tag Removal: SUMO-tag removal is possible
  • Deliverable: Purified recombinant proteins
  • Quality Specification: Typically >90% purity by SDS page
  • Typical Timeline: 8-12 weeks
  •  

For more details, please contact us.

Welcome to contact us or send an email to info@creative-biomart.com for project quotations and more detailed information.
45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.