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Serum-Free Expression System

Due to the many unique advantages of serum-free medium, it has been widely used in recombinant protein expression. Creative BioMart provides serum-free expression system services, from the design of serum-free medium to the production of recombinant proteins using serum-free expression systems, comprehensive services can meet the needs of different customers.

Why Do You Need High-throughput Protein Production?

Why Do You Need High-throughput Protein Production?

Projects such as biomedical research and drug discovery often involve the use of large numbers of proteins produced from different protein families and from different species. Protein expression and purification is a multi-manipulative and time-consuming endeavor whose efficiency greatly affects the progress of these projects. If a high-throughput method is used for protein expression and purification, it is possible to express and purify a large number of these proteins for academic or commercial research in a highly parallel and rapid manner.

Design of Serum-Free Medium

In order to better achieve recombinant protein expression and production, the components of serum-free medium need to be optimized. Energy sources, amino acids, vitamins, lipids, trace elements, inorganic salts, nucleic acids, etc. are all key components involved in the design of serum-free medium. Our expert team has been committed to the research of serum-free expression system. According to different expression objects, we can analyze the nutrients needed in the cell culture process, the changes of cells in the culture process, and the cell growth, so as to design the serum-free medium, and use statistical tools to optimize the medium.

Why Do You Need High-throughput Protein Production?

Service Content of Serum-Free Expression System

  • Serum-Free Medium Development

Medium composition design, commercial medium screening, cell culture medium optimization, scale-up validation

  • Recombinant Protein Expression

Utilize developed or screened serum-free medium for recombinant protein expression

  • Protein Purification

Affinity chromatography, ion exchange chromatography, other purification methods

Why Choose Creative BioMart?

  • No need for serum qualification
  • Increased definition
  • Increased lot-to-lot consistency
  • Simplified purification and downstream processing
  • Better control over the physiological condition of cultures
  • Ability to optimize formulations for specific cell types

The serum-free expression system service provided by Creative BioMart is not only suitable for HEK293 and CHO mammalian expression systems, but also suitable for baculovirus-insect cell expression systems. We will provide personalized services according to different research purposes to ensure that each client can accelerate research progress.

Reference:

  1. Li, W., et al. Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells. Front Bioeng Biotechnol. 2021, 9: 646363.

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