Address: 45-16 Ramsey Road Shirley, NY 11967, USA    USA: 1-631-559-9269   Fax: 1-631-938-8127
UK: 44-207-048-3343  Email: info@creative-biomart.com

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Site-Directed Mutagenesis

Creative Biomart is committed to providing advanced tools for protein expression and purification. As a leading supplier for reagents in the biotechnology field, we understand the importance of convenient and easy-to-use systems for high level expression and sample purification. We invite you to review our growing range of expression systems resulting from our experience in cloning, overexpression and purification.
Site-Directed Mutagenesis
The GenePowerTM site-directed mutagenesis service introduces single or multiple mutations (substitutions, insertions, deletions, or truncations) into existing DNA sequences quickly and economically.

We can replace any protein residue with the residue you specify. A stretch of up to 7 neighboring amino acids can be replaced simultaneously and considered one mutation. Mutations can be introduced in more than one location.

Applications
• Investigate active sites, structure-function relationships, nucleic acid–protein interactions, etc.
• Test functional elements (promoter regions, terminator sequences, etc.).
• Construct fusion proteins and tagged proteins.
• Design knockout constructs.
• Generate alternative splice forms.
• Conduct alanine scans to identify protein functional domains.
Advantages
• Economical alternative to synthetic gene construction for constructs smaller than ~1 kb.
• Mutations exactly where you need them, without the risk of introducing unwanted mutations.
• Unlike "quick change" strategies, the vector is not subjected to PCR—just the insert.
• Insert size can be as large as 6–8 kb.
• No mutations in the vector backbone.
• Mutations can be clustered or far apart, with the same cost and production time.
• Fast turnaround times.
• Template constructs are stored for at least 2 years at no charge and longer-term storage is available.
• Full sequence verification of insert DNA.

 


 

Inquiry
  • 45-16 Ramsey Road, Shirley, NY 11967, USA
    TEL: 1-631-559-9269
    FAX: 1-631-938-8127
    EMAIL: info@creative-biomart.com

    St John's Innovation Centre, Cowley Road, Cambridge CB4 0WS, UK
    TEL: 44-207-048-3343

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