Fluoro Thiol Detection Kit is a fluorescent kit that detects thiol levels in apoptosis, metabolism and oxidative stress in cells or tissue extracts. The kit can be used in the detection of reduced thiols in bacterial, fungal, plant cells and other samples.
During the normal course of metabolism, oxygen is partly reduced as electrons leak out of the electron transport chain during respiration. These partially reduced oxygen species (ROS) can react with organic substances through non-catalytic means. Furthermore, ROS can be generated via endogenous enzyme systems like plasma NADPH oxidase, cytoplasmic xanthine oxidase and organelle sources e.g., cytochrome P-450. ROS have been implicated in regulating diverse cellular functions including proliferation, defense against pathogens, intra-cellular signaling, transcriptional activation and apoptosis. Elevation of ROS beyond the buffering capacity of the cell can lead to oxidative stress. Elevated ROS levels can lead to damage of DNA/RNA, proteins and lipids which may lead to apoptosis. Cells have developed several mechanisms to counter act elevated ROS levels such as a thiol reducing buffer composed of cellular thiol levels (glutathione and thioredoxion) for the maintance of the reduction-oxidation (redox) state of the cell, and enzymes to remove ROS (catalase, superoxide dismutase and glutathione peroxidase).
1. Detection of Reduced thiols in cells or tissue extracts.2. Sensitive fluorescent assay.3. Detection of reduced thiol levels in apoptosis, metabolism and oxidative stress.4. Diverse: detection of reduced thiols in Bacterial, fungal, plant cells or other samples.
1. For Research use only. Not for use in diagnostic procedures.2. Practice safe laboratory procedures by wearing protective clothing and eyewear.3. Keep the dye protected from direct lab lighting.4. Avoid contact with reducing agents.
1. Long Term Storage: Store contents as labeled.2. Upon Arrival: 2-8°C.3. The kit should be stored at 2-8°C until first use.
Reagent-Storage Temperature1. Dye: 1 Vial Dried, 2-8°C;2. Lysis Buffer: 25 mL, 2-8°C;3. GSH Standard: 3 Vials Dried, 2-8°C
Features & Benefits:
1. Detection of reduced Thiols in cells or tissue extracts.2. Sensitive Fluorescent Assay.3. Detection of reduced Thiol levels in apoptosis, metabolism and oxidative stress.4. Detection of reduced Thiols in Bacterial, fungal, plant cells or other samples.5. Fluorescent 96 well Plate Reader Readout (excitation: 488nm and emission at 515-530 nm).