"TPI" Related Products

Triose Phosphate Isomerase Activity Colorimetric Assay Kit

Cat.No.: Kit-0817
Product Overview: Triose Phosphate Isomerase Activity Assay Kit (Colorimetric) is used to measure Triose Phosphate Isomerase activity.
Description: Triose Phosphate Isomerase (TPI or TIM: EC 5.3.1.1) is an important enzyme for glycolysis. It reversibly interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms. In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment. Triose Phosphate Isomerase Activity Assay Kit (Colorimetric) provides a quick and easy way for monitoring Triose Phosphate Isomerase activity in a variety of samples. In this kit, Triose Phosphate Isomerase converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the Enzyme Mix and Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, and high-throughput and can detect Triose Phosphate Isomerase activity as low as 40 mU/mL.
Applications: Measurement of TPI activity in various tissues and cells.Analysis of glycolysis and pentose phosphate pathway and lipid metabolism.
Target Species: Human, Mammals
Usage: For research use only (RUO)
Storage: Store kit at -20°C, protected from light.
Kit Components: TPI Assay Buffer 25 mLTPI Substrate (Lyophilized) 1 vialTPI Enzyme Mix (Lyophilized) 1 vialTPI Developer (Lyophilized) 1 vialNADH Standard (Lyophilized) 1 vialTPI Positive Control (Lyophilized) 1 vial
Detection method: Colorimetric
Compatible Sample Types: Animal tissues: Heart, Kidney, Liver, Muscle, etcCell culture: Adherent or Suspension cellsHuman Serum, Human Plasma

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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