Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > Assay Kits > Other Kits > Urine Albumin-to-Creatinine Ratio Assay Kit

Urine Albumin-to-Creatinine Ratio Assay Kit

"UACR" Related Products.
Product Overview:
UACR Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects albumin (detection range: 0.02- 2.5 mg/ml), creatinine (detection range: 0.002 -0.5 mg/ml) and urine albumin-to-creatinine ratio. The UACR ratio is determined in two steps: First, albumin is determined by using a probe (AB580) that specifically recognizes albumin (Ex/Em = 600/630 nm). Second, creatinine is converted to sarcosine via enzymatic reactions. Sarcosine is specifically oxidized generating a product that reacts with a probe producing a chromophore that can be detected at 570 nm.
100 assays
Urine Albumin-to-Creatinine Ratio (UACR) is one of the two markers used to determine chronic kidney disease (CKD). UACR is recommended to be measured on regular basis on people living with Type I and Type II diabetes. UACR is defined as the ratio between albumin (reported in mg/dl) and creatinine (reported in g/dl). This ratio estimates the amount of albumin excreted in urine during a 24 hr period. Albuminuria is diagnosed when UACR is greater than 30 mg albumin/g creatinine.
Estimation of albumin in biological samplesEstimation of creatinine in biological samplesDetermination of UACR in mammalian urine samples
Store kit at -20°C, protected from light. Briefly spin small vials prior to opening. Read entire protocol before performing the assay.Creatinine Assay Buffer, Albumin Assay Buffer, and Albumin Diluent: Store at -20°C. Bring to room temperature (RT) before use.Albumin Probe (AB580) and Creatinine Probe: Light sensitive. Store at -20°C. Bring to RT before use.Creatinase, Creatininase, and Creatinine Enzyme Mix: Reconstitute with 220 µl of Creatinine Assay Buffer. Aliquot and store at - 20°C. Freeze/thaw should be limited to one time. Keep on ice during use.BSA Standard (2 mg/ml): Store at RT.Creatinine (10 μmol): Reconstitute with 115 µl of dH2O to generate 10 µg/µl Creatinine Standard. Dissolve completely. Store at -20°C. Use within 2 months.
Kit Components:
Creatinine Assay Buffer: 25 mlAlbumin Assay Buffer: 7 mlAlbumin Diluent: 7 mlAlbumin Probe (AB580): 0.4 mlCreatinine Probe: 0.2 mlCreatinase: 1 vialCreatininase: 1 vialCreatinine Enzyme Mix: 1 vialBSA Standard (2 mg/ml): 1 mlCreatinine Standard (10 μmol): 1 vial
Detection method:
Fluorescence (Ex/Em = 600/630 nm) & Absorbance (OD 570 nm)
Compatible Sample Types:
• Albumin: urine, saliva, etc.• Creatinine: urine, serum, etc.• UACR: urine
Features & Benefits:
• Simple & Sensitive• High-Throughput adaptable• Suitable for detecting albumin (detection range: 0.02- 2.5 mg/ml), creatinine (detection range: 0.002 -0.5 mg/ml) and urine albumin-to-creatinine ratio (UACR)

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.