β-N-Acetylglucosaminidase Colorimetric Activity Assay Kit

• Cat.No.: Kit-2149
• Product Overview: NAG Activity Assay Kit provides a simple and sensitive method for monitoring NAG enzymatic activity. In this assay, NAG uses a synthetic pnitrophenol derivative (R-pNP) as a NAG substrate and releases pNP which can be measured at absorbance (OD 400 nm). The assay can detect as low as 50 µU of NAG activity in a variety of samples.

Specification

• Size: 100 assays
• Applications: Measurement of NAG in biological samples from different mammalian species
• Storage: Store kit at -20°C, protected from light. Briefly spin small vials prior to opening. Read entire protocol before performing the assay.NAG Assay Buffer: Bring to room temperature (RT) before use. Store at 4°C or -20°C.NAG Substrate: Light sensitive. Store at -20°C. Once opened, use within two months. Bring to RT before use. Mix well. If precipitation is observed, sonicate the contents in a water bath sonicator (interval: 2 min). Repeat if necessary.p-Nitrophenol (pNP): Light sensitive. Bring to RT before use. Store at -20°C. Once opened, use within two months.NAG Stop Solution: Bring to RT before use. Store at -20 °C. Once opened, use within two months.NAG Positive Control: Reconstitute with 40 µl NAG Assay Buffer. Mix well. Store at -20°C. Use within two months.