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Home > >> Services > cAMP Accumulation Assay

cAMP Accumulation Assay

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G protein-coupled receptors (GPCRs) are a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and cellular responses. There are two principal pathways: cAMP and phosphatidylinositol.

cAMP Accumulation Assay

Creative BioMart offers cAMP Accumulation Assay for measuring cyclic AMP. Creative BioMart has developed the platform optimized for the detection of a specific range of cAMP concentrations, to enable assay sensitivity fine tuning. It gives high quality, cell-based assay screening in 96-, 384- or 1536-well formats .

Features
  • Cell-based functional assay
  • Second messenger accumulation assay
  • Assay formats 100 μL and 20 μL
  • Miniaturization down to < 10μl
  • Compatible with fresh or frozen cells
Applications
  • Gs or Gi coupled receptor activation
  • Assess agonists, antagonists, inverse agonists and allosteric modulators
  • Phosphodiesterase (PDE) monitoring
  • Adenylate cyclase activity assessment

 

Detection of cAMP is based on the competition between cAMP produced by cells and a biotinylated cAMP probe that is recognized by the streptavidin-Donor and anti-cAMP conjugated Acceptor beads. The beads are brought into proximity and a signal is detected. Increased intracellular concentrations of cAMP following Gs coupled GPCR activation by an agonist results in the displacement of the biotinylated cAMP probe and leads to a proportional signal decrease. The effect of antagonists and reverse agonists can similarly be detected. Gi coupled receptor activation can be detected after prestimulating cells with forskolin.

Welcome to contact us or send an email to info@creative-biomart.com for project quotations and more detailed information.
45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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