Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Metabolism Kits > Cholesterol/Cholesteryl Ester Quantitation Kit II

Cholesterol/Cholesteryl Ester Quantitation Kit II

"Cholesterol" Related Products.
Product Overview:
Cholesterol/Cholesteryl Ester Quantitation Kit II is a quantitative colorimetric determination of cholesterol and cholesteryl ester at 450 nm.
Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions. In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm. The assay can detect freeor total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in this kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.
Target Species:
For research use only (RUO)
Store kit at -20°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Keep enzymes and cholesterol standard on ice while using. Read the entire protocol before perform the assay.
Kit Components:
Cholesterol Assay Buffer. 25 mlSubstrate Mix (lyophilized). 1 vialEnzyme Mix (lyophilized). 1 vialEsterase (lyophilized). 1 vialCholesterol Standard (2μg/μl). 100μl
Detection method:
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Culture Medium, Plasma, Serum, Tissue Culture Supernatant, Urine

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.