Continuous PRPP-S Assay Kit

• Cat.No.: Kit-0871
• Product Overview: PRPP-S Assay Kit is designed to measure PRPP (α-D-5-phosphoribosyl-1-pyrophosphate) content in samples. This enzymatic assay is based on a coupled reaction involving Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and Inosine Monophosphate Dehydrogenase (IMPDH).
  The principle of the assay is based on the coupling of the following enzymatic reactions:
  (1) In the presence of ATP and P-ribose, PRPP-Synthetase enzyme catalyzes the formation of PRPP.
  (2) In the presence of Hypoxanthine (Hx), PRPP is converted to IMP by Hypoxanthine-guanine phosphoribosyltransferase (HGPRT).
  (3) IMP is immediately oxidized by a highly active IMPDH in the presence of NAD with simultaneous formation of NADH2 directly monitored spectrophotometrically at 340nm.
  The assay is developed for measuring PRPP-S activity in vitro or in cell lysates.
  For maximal accuracy, the assays with cell lysates are run with and without P-ribose in parallel. The absorbance rate observed in the absence of inosine is used as blank and is subtracted from the absorbance rate measured in its presence.

Specification

• Storage: Once dissolved, the reagents provided in the kit are not stable and should be stored on ice and used the day of preparation.
• Size: 24 analysis