Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human Fibroblast Growth Factor 7, His-tagged

Recombinant Human Fibroblast Growth Factor 7, His-tagged

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"FGF7" Related Products.
Cat. No.:
FGF7-682H
Product Overview:
Recombinant human FGF7contains 164 amino acids and a 16 aa His-tag. Total length is 180 aa.
Description:
The protein encoded bythis gene is a member of the fibroblast growth factor (FGF) family. FGFfamily members possess broad mitogenic and cell survival activities, and areinvolved in a variety of biological processes, including embryonicdevelopment, cell growth, morphogenesis, tissue repair, tumor growth andinvasion. This protein is a potent epithelial cell-specific growth factor,whose mitogenic activity is predominantly exhibited in keratinocytes but notin fibroblasts and endothelial cells.
Source:
Hordeum vulgare(barley grain)
Species:
Human
Form:
Lyophilized from a0.2 µm sterile filtered solution of PBS, pH 7.2.
Molecular Weight:
Predicted MW 21.2kDa including his-tag. The glycosylated recombinant protein migrates with anapparent MW 30 kDa in SDS-PAGE.
Purity:
> 98% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction.
Endotoxin Level:
< 0.005 ng perµg of product (0.05 EU/µg) as measured by turbidimetric kinetic assay.
Biological Activity:
Bioactivity isassayed by measuring the dose dependent effect of human FGF7 on proliferationof mouse BaF3. The ED50 for a typical batch of recombinant human FGF7is 0.20 ng/ml.
Specific Activity:
5.0 x 106U/mg
Cross-Reactivity:
Considerablespecies cross-reactivity, including with mouse, monkey, and pig cells.
Reconstitution:
Centrifuge vialprior to opening. First addsterile distilled water to the vial to fully solubilize the protein to aconcentration not less than 100 µg/ml. After complete solubilization of theprotein, it can be further diluted to other aqueous solutions.
Storage:
The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
OfficialSymbol:
FGF7


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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