| Cat. No. : |
Jag2-5666M |
| Description : |
MouseJag2 is a 150 kDa member of the Delta-Serrate-Lag-2 (DSL) family of ligandsthat activate LIN12/Notch proteins, and thereby regulate cell fatedeterminations during development. It is a type 1 transmembrane protein thatis synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signalsequence, a large, 1059 aa extracellular region, a 21 aa transmembraneregion, and a short, 144 aa cytoplasmic region. The extracellular regioncontains four potential N-linked glycosylation sites, a DSL domain, 16EGF-like repeats, many of which are also sites of calcium binding, and acysteine-rich region just proximal to the transmembrane segment. |
| Source : |
Mammalian cells |
| Form : |
Lyophilizedfrom a 0.2 μm filtered solution in PBS and EDTA. |
| N-terminalSequence : |
Met27 |
| MolecularWeight : |
140.8 kDa (monomer) |
| Activity : |
Measuredby the ability of immobilized protein to enhance BMP-2 induced alkalinephosphatase activity in C3H10T1/2 mouse cells. The ED50 for this effect istypically 0.3-1.2 μg/mL. Measured by its ability to bind rrNotch-1 in afunctional ELISA. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
160-165kDa, reducing conditions. |
| Purity : |
>90%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
100 μg/mLin sterile PBS |
| Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution. |
