Address: 45-16 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
"Jag2" Related Products.
By Species
Human(6)
Mouse(7)
By Conjugate
Fc(4)
His(2)
Home > Products > Recombinant Proteins > Recombinant Mouse Jagged 2, Fc-tagged

Recombinant Mouse Jagged 2, Fc-tagged

  Download Datasheet | See All Jag2 Proteins
Bookmark and Share

Product Description

Cat. No.

Jag2-5666M

Product Overview

RecombinantMouse Jag2 protein was expressed in Murine myeloma cell line with theFc part of Mouse IgG2A Glu98-Lys330 (C-terminus) and Mouse Jag2 Met27-Lys1084(N-terminus).

Description

MouseJag2 is a 150 kDa member of the Delta-Serrate-Lag-2 (DSL) family of ligandsthat activate LIN12/Notch proteins, and thereby regulate cell fatedeterminations during development. It is a type 1 transmembrane protein thatis synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signalsequence, a large, 1059 aa extracellular region, a 21 aa transmembraneregion, and a short, 144 aa cytoplasmic region. The extracellular regioncontains four potential N-linked glycosylation sites, a DSL domain, 16EGF-like repeats, many of which are also sites of calcium binding, and acysteine-rich region just proximal to the transmembrane segment.

Source

Murine myeloma cellline, NS0-derived.

Form

Lyophilizedfrom a 0.2 μm filtered solution in PBS and EDTA.

N-terminalSequence

Met27

MolecularWeight

140.8 kDa (monomer)

Activity

Measuredby the ability of immobilized protein to enhance BMP-2 induced alkalinephosphatase activity in C3H10T1/2 mouse cells. The ED50 for this effect istypically 0.3-1.2 μg/mL. Measured by its ability to bind rrNotch-1 in afunctional ELISA.

Endotoxin Level

<1.0EU per 1μg of the protein by the LAL method.

SDS-PAGE

160-165kDa, reducing conditions.

Purity

>90%,by SDS-PAGE under reducing conditions and visualized by silver stain.

Reconstitution

100 μg/mLin sterile PBS

Storage

Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

Gene Name

Jag2 jagged 2 [ Mus musculus]

Official Symbol

Jag2

Synonyms

Jag2; jagged 2; sm; Serh;D12Ggc2e; mJagged2-1; protein jagged-2; syndactylism

GeneID

16450

mRNA Refseq

NM_010588

Protein Refseq

NP_034718

UniProt ID

Q9QYE5

Chromosome Location

12

Pathway

Delta-NotchSignaling Pathway; Notch Signaling Pathway; Notch receptor binds with aligand; Notch signaling pathway; Receptor-ligand binding initiates the secondproteolytic cleavage of Notch receptor; Signal Transduction; Signaling byNotch

Function

Notch binding;growth factor activity




Download Datasheet:


Online Inquiry    
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.

Recommendation for the product

45-16 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2015 Creative Biomart. All Rights Reserved.