| Cat. No. : |
Gdf9-726M |
| Description : |
Gdf9 is a member ofthe transforming growth factor β (TGF-β) superfamily, and is an oocytesecreted paracrine factor essential for mammalian ovarian folliculogenesis.Mouse Gdf9 is synthesized as a 441 amino acid (aa) prepropeptide thatcontains a 29 aa signal sequence, a 277 aa propeptide, and a 135 aa maturechain). Residues 340-441 constitute a TGF-β like domain. In addition, thereis one potential site of N-linked glycosylation in the mature chain. |
| Source : |
Chinese HamsterOvary cell line, CHO-derived. |
| Form : |
Lyophilized from a0.2 μm filtered solution in HCl with BSA as a carrier protein. |
| N-terminalSequence : |
Gly307 |
| Molecular Weight : |
15.6 kDa |
| Activity : |
Measuredby its ability to induce Smad2 phosphorylation in P19 mouse embryonalcarcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653. 100ng/mL rmGdf9 can effectively induce Smad2 phosphorylation. |
| Endotoxin Level : |
<1.0 EUper 1 μg of the protein by the LAL method. |
| SDS-PAGE : |
20 kDa,reducing conditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
100μg/mL in 4 mM HCl that containing 0.1% bovine serum albumin. |
| Storage : |
Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution. |
