Rabbit monoclonal antibody to Human bone marrow stromal cell antigen 1.
Human CD157, also known as bone marrow sromal cell antigen 1(BST-1), is a pleiotropic ectoenzyme which belongs to the CD38 family and to the growing number of leukocyte surface molecules known to act independently as both receptors and enzymes. As a 45-kDa surface glycoprotein with a GPI anchor, the CD157 molecule displays two distinct domains in its extracellular component. The first is implicated in the enzymic activities of the molecule and the second features adhesion/signalling properties. CD157 shares several characteristics with CD38, including a similar amino acid sequence and enzymic functions. Both molecules are involved in the metabolism of NAD+, and the CD157 gene is synthenic on 4p15 with CD38, with which it also shares a unique genomic organization. Their conservation in phylogeny is striking evidence for their relevance in the life and death cycle of the cell.
Human CD157 / BST1. No cross-reactivity in ELISA with Mouse BST1, Human cell lysate (293 cell line).
Recombinant Human CD157 / BST1 Protein
Western Blot; ELISA
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human BST1 in WB. Using a DAB detection system, the detection limit for Human BST1 is approximately 0.5 ng/lane under non-reducing condition. Use of this antibody under reducing conditions is not recommended.Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect Human BST1. The detection limit for Human BST1 is approximately 0.0049 ng/well.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human CD157 / BST1.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.